The impact of artificial intelligence (AI) on our lives is incredible and what may seem like just a reduction in task completion time is one clear aspect of its influence. Using AI involves more than just quick data assessment; it means employing algorithms capable of independent processing, learning, and knowledge generation. Thus, humanity possesses a powerful tool that must be used responsibly and ethically. Pharmacological research, for instance, relies on animal models for preliminary testing before clinical trials. Guiding this testing are the 3Rs principles aiming to reduce, refine, and replace animal models when possible, for more ethical and advanced research. In this article, we will explore how AI can decrease the need for animal use in research and its potential impact on public acceptance.